Allied Market Research

2024

Isothermal Amplification Market

Isothermal amplification market: Global Opportunity Analysis and Industry Forecast, 2024-2031 by Technology (NASBA, HDA, LAMP, SPIA, SDA, NEAR, TMA, SMAP2, RCA, RPA, Others), by Application (Blood screening, Infectious disease diagnostics, Cancer, Others) and by End User (Hospitals, Diagnostics, Central and reference labs, Others): Global Opportunity Analysis and Industry Forecast, 2023-2032

LS : Medical Devices & Supplies

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Author's: | Roshan Deshmukh
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Isothermal Amplification Market Outlook 2027

In order to study individual genes or specific DNA regions of interest, it is often necessary to obtain a large quantity of nucleic acid for study. Rather than isolate a single copy of the target DNA from a large number of cells, it is often more useful to generate multiple copies of a target from a single molecule of DNA or mRNA, via an in vitro amplification method. Isothermal amplification methods provide detection of a nucleic acid target sequence in a streamlined, exponential manner, and are not limited by the constraint of thermal cycling. Although these methods can vary considerably, they all share some features in common. For example, because the DNA strands are not heat denatured, all isothermal methods rely on an alternative approach to enable primer binding and initiation of the amplification reaction: a polymerase with strand-displacement activity. Once the reaction is initiated, the polymerase must also separate the strand that is still annealed to the sequence of interest. Isothermal amplification chemistry has been applied to diagnostics with great success and is utilized in several commercial molecular diagnostic platforms, serving large testing centers and point-of-care markets. Isothermal Amplification is becoming more and more popular as a simple and cost effective alternative to standard PCR.

Covid-19 Scenario analysis:

The novel coronavirus (SARS-CoV-2; coronavirus disease 2019, COVID-19) has recently emerged, rapidly spreading and severely straining the capacity of the global health community. Many nations are employing combinations of containment and mitigation strategies, where early diagnosis of COVID-19 is vital in controlling illness progression and limiting viral spread within the population. Thus, rapid and accurate methods of early detection are vital to contain COVID-19 and prevent further spread and predicted subsequent infectious waves of viral recurrence in future. Immediately after its initial characterization, Chinese and American Centers for Disease Control and Prevention (CDCs) rapidly employed molecular assays for detection of COVID-19, mostly employing real-time polymerase chain reaction (RT-PCR) methods. However, such methods require specific expensive items of equipment and highly trained analysts, requiring upwards of 4–8 h to process. These requirements coupled with associated financial pressures may prevent effective deployment of such diagnostic tests. Loop mediated isothermal amplification (LAMP) is method of nucleic acid amplification which exhibits increased sensitivity and specificity are significantly rapid, and do not require expensive reagents or instruments, which aids in cost reduction for coronavirus detection. Thus, the isothermal amplification market is growing during pandemic.

Top impacting factors: Market Scenario Analysis, Trends, Drivers and Impact analysis:

Drivers:

Increase in Viral diseases:

Viral disease emergence represents a serious threat to global public health. Indeed, several viral epidemics over the past few decades have emerged with increasing frequency, including the severe acute respiratory syndrome coronavirus (SARS-CoV), H1N1 influenza, and more recently the Middle East respiratory syndrome coronavirus (MERS-CoV). Thus, rapid and accurate methods of early detection are vital to contain viral infections, and prevent further spread and predicted subsequent infectious waves of viral recurrence in future. Loop mediated isothermal amplification (LAMP) is a novel isothermal nucleic acid amplification method. LAMP exhibits increased sensitivity and specificity due to an exponential amplification feature that utilises 6 different target sequences simultaneously identified by separate distinct primers in the same reaction. LAMP assays are significantly rapid, and do not require expensive reagents or instruments, which aids in cost reduction for coronavirus detection. Current improved LAMP assays employ a total of six primers, recognising eight distinct sites of the target sequence. A strand-displacing DNA polymerase is employed to initiate synthesis, while two primers form loop structures to facilitate and accelerate subsequent rounds of amplification. Specifically, LAMP employs two inner primers (FIP and BIP, which in turn consist of two parts each) and two outer primers (F3 and B3 which can recognize a total of six distinct regions within the target DNA). Two loop primers are employed (Forward loop primer; LF, and backward loop primer; LB) to accelerate amplification and detection efficiency. 

Growth in prevalence cancer:

Increasing population suffering from cancer and infectious diseases has boosted demand for low-cost diagnostics such as INAAT. Considering the high morbidity and metastasis of tumor malignancy, early and accurate detection of the survivin gene plays a pivotal role in anticancer therapies. The loop-mediated isothermal amplification (LAMP) assay strategy was demonstrated by using optimized real-time fluorescence measurements in a single tube at a constant temperature of 62 °C for 30–60 min. The survivin gene was the target sequence amplified by LAMP. By testing serial tenfold dilutions of target DNA ranging from 1 × 106 copies to 1 copy per tube, the developed method showed that the detection limit (analytical sensitivity) for the survivin gene was 10 copies per tube. Our assay strategy is able to offer high amplification efficiency, excellent reproducibility, and cost-effective and simplified operations. Without the requirements for sophisticated equipment and technical skills of the PCR, the LAMP method can add a great deal of value to small-scale hospitals, point-of-care testing, and clinical laboratories. Thus the growing prevalence of Cancer will boost the diagnostic market of Isothermal amplification.

Restrains:

Because of the high cost, the technique has witnessed limited adoption in developing countries, whilst the demand growing, especially for clinical application. 

Key benefits of the report:

  • This study presents the analytical depiction of the global Isothermal amplification industry along with the current trends and future estimations to determine the imminent investment pockets.
  • The report presents information related to key drivers, restraints, and opportunities along with detailed analysis of the global Isothermal amplification market share.
  • The current market is quantitatively analyzed to highlight the global Isothermal amplification market growth scenario.
  • Porter’s five forces analysis illustrates the potency of buyers & suppliers in the market. 
  • The report provides a detailed global Isothermal amplification market analysis based on competitive intensity and how the competition will take shape in coming years. 

Questions answered in the Isothermal amplification Market research report:

  • Who are the leading market players active in the Isothermal amplification market?
  • What the current trends will influence the market in the next few years?
  • What are the driving factors, restraints, and opportunities in the market?
  • What are the projections for the future that would help in taking further strategic steps?

Isothermal amplification market: Global Opportunity Analysis and Industry Forecast, 2024-2031 by Technology Report Highlights

Aspects Details
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By Technology
  • NASBA
  • HDA
  • LAMP
  • SPIA
  • SDA
  • NEAR
  • TMA
  • SMAP2
  • RCA
  • RPA
  • Others
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By Application
  • Blood screening
  • Infectious disease diagnostics
  • Cancer
  • Others
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By End User
  • Hospitals
  • Diagnostics
  • Central and reference labs
  • Others
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By Region
  • North America  (U.S., Canada, Mexico)
  • Europe  (France, Germany, Italy, Spain, UK, Rest of Europe)
  • Asia-Pacific  (China, Japan, India, South Korea, Australia, Rest of Asia-Pacific)
  • LAMEA  (Brazil, South Africa, Saudi Arabia, Rest of LAMEA)
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Key Market Players

DiaSorin S.p.A.,, bioMérieux SA,, Grifols, S.A.,, Tecan Trading AG,, Abbott Laboratories, Inc.,, Quidel Corporatio,, Hologic, Inc.,, EIKEN CHEMICAL CO., LTD.,, Meridian Bioscience,, Becton, Dickinson & Company,, QIAGEN,

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Isothermal amplification market: Global Opportunity Analysis and Industry Forecast, 2024-2031 by Technology

Global Opportunity Analysis and Industry Forecast, 2020-2027 by Technology (NASBA, HDA, LAMP, SPIA, SDA, NEAR, TMA, SMAP2, RCA, RPA, Others), by Application (Blood screening, Infectious disease diagnostics, Cancer, Others) and by End User (Hospitals, Diagnostics, Central and reference labs, Others): Global Opportunity Analysis and Industry Forecast, 2023-2032